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Expression of Tuftelin in Human Dental Germs. Correspondencia a :. Its function continues unclarified, although it plays a role in the biomineralization of the dental organ. With the present studio the location was identified in the different structures of dental germs from human fetuses, according to the results it was observed the expression in the pre-secretor stage being observed in the cytoplasm of ameloblasts, stellate reticulum, dental papilla, also in the intermediate stratum; in the secretor it was mainly identified in the amelodentinal junction and in the outer surface of enamel, observing a marked expression of the protein in the basal portion of the odontoblastic process, but not in the extracellular matrix of the dentine.
According to the results obtained it can be considered that its expression occurs in both amelogenesis and odontegenesis in unmineralized tissues. La mayor parte de los estudios realizados previamente se han realizado en modelos animales.
Luo et al. Obteniendo un total de 20 muestras para analizar. Las figuras 1a y 1c se tomaron a 40x y la 1b y 1 d a x. Las Figuras 1a , 1c y 1e se encuentran a 40x y las 1b, 1d y 1f a x. En esta etapa no se observa presencia en la papila Fig. Deutsch et al. Avery, J. Oral Development and Histology. Stuttgart, Thieme, Current knowledge of tooth development: patterning and mineralization of the murine dentition. Deutsch, D. The human tuftelin gene and the expression of tuftelin in mineralizing and nonmineralizing tissues.
Tissue Res. Oral Sci. The enamelin tuftelin gene. Tuftelin--aspects of protein and gene structure. Diekwisch, T. Immunohistochemical similarities and differences between amelogenin and tuftelin gene products during tooth development.
Fincham, A. The structural biology of the developing dental enamel matrix. Amelogenin and enamelysin localization in human dental germs. In Vitro Cell. Expression of Mmp in dental germs of human fetus. Jeremias, F. Genes expressed in dental enamel development are associated with molar-incisor hypomineralization. Oral Biol. Hulley, E. Baltimore, Williams and Wilkins Co. Lacruz, R. Regulation of pH during amelogenesis. Tissue Int. Leiser, Y. Localization, quantification, and characterization of tuftelin in soft tissues.
Hoboken , 5 , Luo, W. In vivo overexpression of tuftelin in the enamel organic matrix. Cells Tissues Organs, 4 , MacDougall, M. Cloning, characterization, and tissue expression pattern of mouse tuftelin cDNA. Mao, Z. The human tuftelin gene: cloning and characterization. Gene, 2 , Mihu, C. Tooth enamel, the result of the relationship between matrix proteins and hydroxyapatite crystals.
Paine, M. Protein-to-protein interactions: criteria defining the assembly of the enamel organic matrix. Satchell, P. Conservation and variation in enamel protein distribution during vertebrate tooth development. Part A Ecol. Shay, B. High yield expression of biologically active recombinant full length human tuftelin protein in baculovirus-infected insect cells.
Protein Expr. Simmer, J. Molecular mechanisms of dental enamel formation. Expression, structure, and function of enamel proteinases. Tariq, A. Association of the use of bacterial cell wall synthesis Inhibitor drugs in early childhood with the Developmental Defects of Enamel. Zeichner-David, M. Timing of the expression of enamel gene products during mouse tooth development.
Is there more to enamel matrix proteins than biomineralization? Matrix Biol. Email: pacogtz uaslp. Recibido : Aceptado: Servicios Personalizados Revista. Casilla D Temuco - Chile Tel.
Los grupos de edad para cada sexo, fueron conformados de tal manera que cada grupo estuvo constituido por al menos 10 individuos, con diferencias de 11 meses entre estos. Las puntuaciones obtenidas fueron sumadas y el resultado transformado en ED por medio de las tablas estandarizadas para cada sexo. Chicago, Ill, USA. A new system of dental age assessment. Human Biol. Dental age estimation in Spanish and Venezuelan children.